Gene expression analysis by quantitative reverse transcription PCR (qRT–PCR) allows accurate quantifications of messenger RNA (mRNA) levels over different samples. Create two targets with the same SYBR DYE; Name your GENE (red) and REFERENCE gene (Green: GAPDH or Beta Actin) Use 2 (two) separate reaction tubes with the same sample name for […]
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How to set ip address of computer to use Optimal Software?
To be able to use optimal control and analysis software, ip settings of the computer has to be done (no need to change ip of the instrument), please close the Wi/Fi (or all other internet connections) before starting this process, please note that Wi-Fi must be turned off while the […]
Read MoreHow to install Optimal Software to your computer?
To be able to use optimal control and analysis software, ip settings of the instrument has to be done, please close the Wi/Fi (or all other internet connections) before starting this process, please note that Wi-Fi must be turned off while the device is running as well; Optimal software can […]
Read MoreHow to export your result with Optimal Software?
LongGene Optimal is a functional control and analysis software that you can use it to create and use your protocols, analysis your results, also export your run datas. Related Links;
Read MoreHow to create a protocol with Optimal Software?
Optimal uses the special *.txc files, you can create a new one or you can use Template button to select your created (ready to use) protocols for the quick setup. Please close the connection between computer and instrument to be able to create a ready to use protocol correctly. After […]
Read MoreRT-PCR Result: Normal Amplification
This is an expected Real Time PCR results because; (FAM: Specific regions for Covid-19 virus, HEX: Internal Control, RP gene for extraction) Positive control (PC) is amplified normally (This proves that PCR is done, instrument and kit is working properly) Negative Control (NC) has no amplification, so it proves that […]
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