How to do a self-test to check if there is a problem?

Achieving accurate and reliable results depends on: diagnostic kit, RNA prep solution, swap for sampling, disposables (pipette tips, strip tubes and plates for RT-PCR) and instrument.

Outstanding Features of the LongGene devices

• Using LED light sources for each channel separately (No optical calibration is needed)
• Using new generation SSLP CCD cameras each channel separately (No optical calibration is needed)
• Using new generation peltier elements (Supports fast protocols, 1,000,000 cycle life time)
• Self test function

How to do a self-test function of the device to check if there is a problem with the instrument?

Please remove all sample tubes from the sample block and follow the below steps;

Conclusion

If the self-test has passed all the steps with green ‘OK’, it proves that there is no technical problem with your device, please search for the problem in the above possibilities that we listed below;

Possible Reasons of Poor Results;

• Not to store IVD kit at -20 C in a deep freezer (Please also check the expiration date)
• Using IVD kit after freeze and defrost more than 2 times
• Low quality RNA Prep solutions (Please check the expiration date)
• Low quality white Sample tubes (Strip tubes, optical caps, plates or sealing)
• Not doing master mix and sample pipetting on a bench top cooler
• Pipetting master mix and samples under a direct light source (Taqman probes may be affected negatively)
• Using unfiltered, non-sterile and non-hydrophobic pipette tips
• Using RT-PCR at too hot or too low room temperatures