Achieving accurate and reliable results depends on: diagnostic kit, RNA prep solution, swap for sampling, disposables (pipette tips, strip tubes and plates for RT-PCR) and instrument. 

Possible Reasons of Poor Results;

  • Not to store IVD kit at -20 C in a deep freezer (Please check the expiration date)
  • To use the IVD kit after freeze and defrost more than 2 times
  • Low quality RNA Prep solutions (Please check the expiration date)
  • Low quality white Sample tubes (Strip tubes, optical caps, plates or sealing)
  • Not doing master mix and sample pipetting on a bench top cooler
  • Pipetting master mix and samples under a direct light source (Taqman probes may be affected negatively)
  • Using unfiltered, non-sterile and non-hydrophobic pipette tips
  • Using RT-PCR at too hot or too low room temperatures

Main Features of the LongGene devices

  • Using LED light sources for each channel separately (No optical calibration is needed)
  • Using new generation SSLP CCD cameras each channel separately (No optical calibration is needed)
  • Using new generation peltier elements (Supports fast protocols, 1,000,000 cycle life time)
  • Self test function
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Expected Sigmoidal Amplification Curves (PC and green EC curves)

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