{"id":353,"date":"2021-09-19T12:21:19","date_gmt":"2021-09-19T12:21:19","guid":{"rendered":"http:\/\/longgene.com.tr\/?p=353"},"modified":"2021-10-10T13:32:11","modified_gmt":"2021-10-10T13:32:11","slug":"result-interpretation","status":"publish","type":"post","link":"https:\/\/longgene.com.tr\/?p=353","title":{"rendered":"RT-PCR Result: Normal Amplification"},"content":{"rendered":"\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" width=\"918\" height=\"662\" src=\"http:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/09\/plot_normal.png\" alt=\"\" class=\"wp-image-357\" srcset=\"https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/09\/plot_normal.png 918w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/09\/plot_normal-300x216.png 300w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/09\/plot_normal-768x554.png 768w\" sizes=\"(max-width: 918px) 100vw, 918px\" \/><\/figure>\n\n\n\n<p>This is an expected Real Time PCR results because;<\/p>\n\n\n\n<p>(<strong>FAM:<\/strong> Specific regions for Covid-19 virus, <strong>HEX:<\/strong> Internal Control, RP gene for extraction)<\/p>\n\n\n\n<ul><li><strong>Positive control (PC)<\/strong> is amplified normally (This proves that PCR is done, instrument and kit is working properly)<\/li><li><strong>Negative Control (NC)<\/strong> has no amplification, so it proves that there is no contamination in your master mixes.<\/li><li>Amplification curves are <strong>sigmoidal <\/strong>as expected (Some positive samples may give a Ct but it may not be a sigmoidal curve)<\/li><li>All tubes has internal control <strong>(RP, IC, IPC)<\/strong> amplification curves (green), that proves that <strong>sampling <\/strong>is done correctly.  <\/li><\/ul>\n\n\n\n<hr class=\"wp-block-separator is-style-wide\"\/>\n\n\n\n<p><strong>Related Links;<\/strong><\/p>\n\n\n\n<div class=\"wp-block-buttons\">\n<div class=\"wp-block-button has-custom-font-size has-small-font-size\"><a class=\"wp-block-button__link\" href=\"http:\/\/longgene.com.tr\/?page_id=211\">96 WELL Real t\u0131me PCR<\/a><\/div>\n\n\n\n<div class=\"wp-block-button has-custom-font-size has-small-font-size\"><a class=\"wp-block-button__link\" href=\"http:\/\/longgene.com.tr\/?page_id=222\">48 well Real t\u0131me PCR<\/a><\/div>\n\n\n\n<div class=\"wp-block-button has-custom-font-size has-small-font-size\"><a class=\"wp-block-button__link\" href=\"http:\/\/longgene.com.tr\/?page_id=227\">16 well real t\u0131me pcr<\/a><\/div>\n\n\n\n<div class=\"wp-block-button has-custom-font-size has-small-font-size\"><a class=\"wp-block-button__link\" href=\"http:\/\/longgene.com.tr\/?page_id=204\">pcr d\u0131sposables<\/a><\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>This is an expected Real Time PCR results because; (FAM: Specific regions for Covid-19 virus, HEX: Internal Control, RP gene for extraction) Positive control (PC) is amplified normally (This proves that PCR is done, instrument and kit is working properly) Negative Control (NC) has no amplification, so it proves that [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5,7],"tags":[],"_links":{"self":[{"href":"https:\/\/longgene.com.tr\/index.php?rest_route=\/wp\/v2\/posts\/353"}],"collection":[{"href":"https:\/\/longgene.com.tr\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/longgene.com.tr\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/longgene.com.tr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/longgene.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=353"}],"version-history":[{"count":8,"href":"https:\/\/longgene.com.tr\/index.php?rest_route=\/wp\/v2\/posts\/353\/revisions"}],"predecessor-version":[{"id":406,"href":"https:\/\/longgene.com.tr\/index.php?rest_route=\/wp\/v2\/posts\/353\/revisions\/406"}],"wp:attachment":[{"href":"https:\/\/longgene.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=353"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/longgene.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=353"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/longgene.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=353"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}