{"id":369,"date":"2021-09-19T13:05:29","date_gmt":"2021-09-19T13:05:29","guid":{"rendered":"http:\/\/longgene.com.tr\/?p=369"},"modified":"2021-10-10T13:37:20","modified_gmt":"2021-10-10T13:37:20","slug":"rt-pcr-result-no-amplification","status":"publish","type":"post","link":"https:\/\/longgene.com.tr\/?p=369","title":{"rendered":"RT-PCR Result: No Amplification"},"content":{"rendered":"\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"1024\" height=\"511\" src=\"http:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/09\/plot_exist_or_not-1024x511.png\" alt=\"\" class=\"wp-image-370\" srcset=\"https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/09\/plot_exist_or_not-1024x511.png 1024w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/09\/plot_exist_or_not-300x150.png 300w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/09\/plot_exist_or_not-768x383.png 768w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/09\/plot_exist_or_not-1536x766.png 1536w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/09\/plot_exist_or_not-1800x898.png 1800w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/09\/plot_exist_or_not.png 1972w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p>To check whether no amplification is caused by kir or instrument; <\/p>\n\n\n\n<ul><li><strong>Deselect<\/strong> the Subtract Baseline and Normalization functions<\/li><li>If the lines are unexpected curves <strong>as above<\/strong> then it proves that instrument is collection data so you have to focus on your protocol or kits<\/li><li>If the there is only a <strong>single line<\/strong> then it means that instrument is not collection data from the samples. <\/li><li>Self Test: Go to main menu on the touch screen then; <strong>Tools\/System Set\/Self Test\/Start <\/strong><\/li><li>Self test takes around 5 minutes and report you the functions of the instruments: <strong>OK <\/strong>(Green, Passed) or <strong>FAIL <\/strong>(Red) <\/li><\/ul>\n\n\n\n<hr class=\"wp-block-separator is-style-wide\"\/>\n\n\n\n<p><strong>Related Links;<\/strong><\/p>\n\n\n\n<div class=\"wp-block-buttons\">\n<div class=\"wp-block-button has-custom-font-size has-small-font-size\"><a class=\"wp-block-button__link\" href=\"http:\/\/longgene.com.tr\/?page_id=211\">96 WELL Real t\u0131me PCR<\/a><\/div>\n\n\n\n<div class=\"wp-block-button has-custom-font-size has-small-font-size\"><a class=\"wp-block-button__link\" href=\"http:\/\/longgene.com.tr\/?page_id=222\">48 well Real t\u0131me PCR<\/a><\/div>\n\n\n\n<div class=\"wp-block-button has-custom-font-size has-small-font-size\"><a class=\"wp-block-button__link\" href=\"http:\/\/longgene.com.tr\/?page_id=227\">16 well real t\u0131me pcr<\/a><\/div>\n\n\n\n<div class=\"wp-block-button has-custom-font-size has-small-font-size\"><a class=\"wp-block-button__link\" href=\"http:\/\/longgene.com.tr\/?page_id=204\">pcr d\u0131sposables<\/a><\/div>\n<\/div>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>To check whether no amplification is caused by kir or instrument; Deselect the Subtract Baseline and Normalization functions If the lines are unexpected curves as above then it proves that instrument is collection data so you have to focus on your protocol or kits If the there is only a [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5],"tags":[9,12,13,19],"_links":{"self":[{"href":"https:\/\/longgene.com.tr\/index.php?rest_route=\/wp\/v2\/posts\/369"}],"collection":[{"href":"https:\/\/longgene.com.tr\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/longgene.com.tr\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/longgene.com.tr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/longgene.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=369"}],"version-history":[{"count":5,"href":"https:\/\/longgene.com.tr\/index.php?rest_route=\/wp\/v2\/posts\/369\/revisions"}],"predecessor-version":[{"id":643,"href":"https:\/\/longgene.com.tr\/index.php?rest_route=\/wp\/v2\/posts\/369\/revisions\/643"}],"wp:attachment":[{"href":"https:\/\/longgene.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=369"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/longgene.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=369"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/longgene.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=369"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}