{"id":871,"date":"2021-10-16T16:26:51","date_gmt":"2021-10-16T16:26:51","guid":{"rendered":"https:\/\/longgene.com.tr\/?p=871"},"modified":"2021-10-17T11:02:33","modified_gmt":"2021-10-17T11:02:33","slug":"how-to-check-the-performance-of-the-thermal-gradient-block","status":"publish","type":"post","link":"https:\/\/longgene.com.tr\/?p=871","title":{"rendered":"How to check the performance of the thermal gradient block?"},"content":{"rendered":"\n<p>Real time PCR instruments has two main functions; thermal block and optical module, new generation peltiers control the heating and cooling sensitivity, LED excitation source and new generation CCD cameras are stated in the optical module. So, to check the performance of a RT-PCR; you have to check the performance of both modules. <\/p>\n\n\n\n<p><strong>Main Features of the LongGene devices<\/strong><\/p>\n\n\n\n<ul><li>Using LED light sources for each channel separately (No optical calibration is needed)<\/li><li>Using new generation Sslp CCD cameras each channel separately (No optical calibration is needed)<\/li><li>Using new generation peltier elements (Supports fast protocols, 1,000,000 cycle life time)<\/li><li>Self test function<\/li><\/ul>\n\n\n\n<p><strong>How to do check the uniformity and accuracy of RT-PCR gradient peltier block? <\/strong><\/p>\n\n\n\n<p>Your instrument has &#8216;Active Temperature Monitoring System&#8217;, so you can do real time sensor (Run Temperature: RunTemp) whenever you like, your the heated lid and peltier sets during the run (of water the run), this will  You can check RunTemp screen during the run or after the run, please follow the below steps;<\/p>\n\n\n\n<div class=\"wp-block-columns\">\n<div class=\"wp-block-column\">\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"1024\" height=\"768\" src=\"https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/st1-1024x768.jpg\" alt=\"q2000, q2000a, q2000b, q2000c, pcr, qpcr, rt pcr, qrt pcr, real time, real time per, thermal cycler, longgene, longGene.com.tr, ivd ce, biotechnology, LongGene europe, europe pcr, disgnostics, snp, absolute quantification, relative quantification, dna, cdna, thermal protocol, LongGene, long gene, end point pcr, covid19, fast pcr, polymerase chain reaction, Q1000, Q1000+, optimal, longgene turkey, LongGene europe, pcr mix, rt-pcr mix, how to prepare pcr mix, 16 well rt pcr, optimal software\" class=\"wp-image-855\" srcset=\"https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/st1-1024x768.jpg 1024w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/st1-300x225.jpg 300w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/st1-768x576.jpg 768w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/st1-1536x1152.jpg 1536w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/st1-2048x1536.jpg 2048w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/st1-2000x1500.jpg 2000w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/st1-1000x750.jpg 1000w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/st1-1800x1350.jpg 1800w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/st1-747x560.jpg 747w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/st1-600x450.jpg 600w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column\">\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"1024\" height=\"768\" src=\"https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_2-1024x768.jpg\" alt=\"q2000, q2000a, q2000b, q2000c, pcr, qpcr, rt pcr, qrt pcr, real time, real time per, thermal cycler, longgene, longGene.com.tr, ivd ce, biotechnology, LongGene europe, europe pcr, disgnostics, snp, absolute quantification, relative quantification, dna, cdna, thermal protocol, LongGene, long gene, end point pcr, covid19, fast pcr, polymerase chain reaction, Q1000, Q1000+, optimal, longgene turkey, LongGene europe, pcr mix, rt-pcr mix, how to prepare pcr mix, 16 well rt pcr, optimal software\" class=\"wp-image-893\" srcset=\"https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_2-1024x768.jpg 1024w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_2-300x225.jpg 300w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_2-768x576.jpg 768w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_2-1536x1152.jpg 1536w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_2-2048x1536.jpg 2048w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_2-2000x1500.jpg 2000w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_2-1000x750.jpg 1000w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_2-1800x1350.jpg 1800w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_2-747x560.jpg 747w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_2-600x450.jpg 600w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns\">\n<div class=\"wp-block-column\">\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"1024\" height=\"768\" src=\"https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_3-1024x768.jpg\" alt=\"q2000, q2000a, q2000b, q2000c, pcr, qpcr, rt pcr, qrt pcr, real time, real time per, thermal cycler, longgene, longGene.com.tr, ivd ce, biotechnology, LongGene europe, europe pcr, disgnostics, snp, absolute quantification, relative quantification, dna, cdna, thermal protocol, LongGene, long gene, end point pcr, covid19, fast pcr, polymerase chain reaction, Q1000, Q1000+, optimal, longgene turkey, LongGene europe, pcr mix, rt-pcr mix, how to prepare pcr mix, 16 well rt pcr, optimal software\" class=\"wp-image-894\" srcset=\"https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_3-1024x768.jpg 1024w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_3-300x225.jpg 300w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_3-768x576.jpg 768w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_3-1536x1152.jpg 1536w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_3-2048x1536.jpg 2048w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_3-2000x1500.jpg 2000w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_3-1000x750.jpg 1000w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_3-1800x1350.jpg 1800w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_3-747x560.jpg 747w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_3-600x450.jpg 600w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-block-column\">\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"1024\" height=\"768\" src=\"https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_4-1024x768.jpg\" alt=\"q2000, q2000a, q2000b, q2000c, pcr, qpcr, rt pcr, qrt pcr, real time, real time per, thermal cycler, longgene, longGene.com.tr, ivd ce, biotechnology, LongGene europe, europe pcr, disgnostics, snp, absolute quantification, relative quantification, dna, cdna, thermal protocol, LongGene, long gene, end point pcr, covid19, fast pcr, polymerase chain reaction, Q1000, Q1000+, optimal, longgene turkey, LongGene europe, pcr mix, rt-pcr mix, how to prepare pcr mix, 16 well rt pcr, optimal software\" class=\"wp-image-895\" srcset=\"https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_4-1024x768.jpg 1024w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_4-300x225.jpg 300w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_4-768x576.jpg 768w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_4-1536x1152.jpg 1536w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_4-2048x1536.jpg 2048w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_4-2000x1500.jpg 2000w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_4-1000x750.jpg 1000w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_4-1800x1350.jpg 1800w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_4-747x560.jpg 747w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/RunTemp_4-600x450.jpg 600w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n<\/div>\n<\/div>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"1024\" height=\"914\" src=\"https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/Oprimal_vs_RunTemp-1024x914.png\" alt=\"q2000, q2000a, q2000b, q2000c, pcr, qpcr, rt pcr, qrt pcr, real time, real time per, thermal cycler, longgene, longGene.com.tr, ivd ce, biotechnology, LongGene europe, europe pcr, disgnostics, snp, absolute quantification, relative quantification, dna, cdna, thermal protocol, LongGene, long gene, end point pcr, covid19, fast pcr, polymerase chain reaction, Q1000, Q1000+, optimal, longgene turkey, LongGene europe, pcr mix, rt-pcr mix, how to prepare pcr mix, 16 well rt pcr, optimal software\" class=\"wp-image-909\" srcset=\"https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/Oprimal_vs_RunTemp-1024x914.png 1024w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/Oprimal_vs_RunTemp-300x268.png 300w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/Oprimal_vs_RunTemp-768x685.png 768w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/Oprimal_vs_RunTemp-1536x1371.png 1536w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/Oprimal_vs_RunTemp-1000x893.png 1000w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/Oprimal_vs_RunTemp-1800x1607.png 1800w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/Oprimal_vs_RunTemp-627x560.png 627w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/Oprimal_vs_RunTemp-600x536.png 600w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/Oprimal_vs_RunTemp.png 1842w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><figcaption>Relationship between Optimal Protocol Page (PC Software) and RunTemp Screen (Instrument)<\/figcaption><\/figure>\n\n\n\n<p><strong>Related Links;<\/strong><\/p>\n\n\n\n<hr class=\"wp-block-separator is-style-wide\"\/>\n\n\n\n<div class=\"wp-block-buttons\">\n<div class=\"wp-block-button has-custom-font-size has-small-font-size\"><a class=\"wp-block-button__link\" href=\"https:\/\/longgene.com.tr\/?p=853\">How to do a self-test to check if there is a problem?<\/a><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-buttons\">\n<div class=\"wp-block-button has-custom-font-size has-small-font-size\"><a class=\"wp-block-button__link\" href=\"https:\/\/longgene.com.tr\/?p=866\">What could be the reason of poor RT-PCR results?<\/a><\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Real time PCR instruments has two main functions; thermal block and optical module, new generation peltiers control the heating and cooling sensitivity, LED excitation source and new generation CCD cameras are stated in the optical module. So, to check the performance of a RT-PCR; you have to check the performance [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[6,35],"tags":[10,15,34,37,38,39],"_links":{"self":[{"href":"https:\/\/longgene.com.tr\/index.php?rest_route=\/wp\/v2\/posts\/871"}],"collection":[{"href":"https:\/\/longgene.com.tr\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/longgene.com.tr\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/longgene.com.tr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/longgene.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=871"}],"version-history":[{"count":7,"href":"https:\/\/longgene.com.tr\/index.php?rest_route=\/wp\/v2\/posts\/871\/revisions"}],"predecessor-version":[{"id":914,"href":"https:\/\/longgene.com.tr\/index.php?rest_route=\/wp\/v2\/posts\/871\/revisions\/914"}],"wp:attachment":[{"href":"https:\/\/longgene.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=871"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/longgene.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=871"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/longgene.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=871"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}