{"id":926,"date":"2021-10-23T10:28:31","date_gmt":"2021-10-23T10:28:31","guid":{"rendered":"https:\/\/longgene.com.tr\/?p=926"},"modified":"2021-10-24T10:17:52","modified_gmt":"2021-10-24T10:17:52","slug":"how-are-gene-expression-relative-quantification-studies-done","status":"publish","type":"post","link":"https:\/\/longgene.com.tr\/?p=926","title":{"rendered":"How are gene expression (Relative Quantification) studies done?"},"content":{"rendered":"\n<p>Gene expression analysis by quantitative&nbsp;reverse transcription PCR&nbsp;(qRT\u2013PCR) allows accurate quantifications of messenger RNA (mRNA) levels over different samples. <\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"1024\" height=\"534\" src=\"https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R1-1024x534.jpg\" alt=\"q2000, q2000a, q2000b, q2000c, pcr, qpcr, rt pcr, qrt pcr, real time, real time per, thermal cycler, longgene, longGene.com.tr, ivd ce, biotechnology, LongGene europe, europe pcr, disgnostics, snp, absolute quantification, relative quantification, dna, cdna, thermal protocol, LongGene, long gene, end point pcr, covid19, fast pcr, polymerase chain reaction, Q1000, Q1000+, optimal, longgene turkey, LongGene europe, pcr mix, rt-pcr mix, how to prepare pcr mix, 16 well rt pcr, optimal software\" class=\"wp-image-928\" srcset=\"https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R1-1024x534.jpg 1024w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R1-300x156.jpg 300w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R1-768x400.jpg 768w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R1-1536x801.jpg 1536w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R1-1000x521.jpg 1000w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R1-1800x939.jpg 1800w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R1-1074x560.jpg 1074w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R1-600x313.jpg 600w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R1.jpg 1910w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><figcaption>Select Experiment Type: Relative Quantification<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"1024\" height=\"530\" src=\"https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R2-1024x530.jpg\" alt=\"q2000, q2000a, q2000b, q2000c, pcr, qpcr, rt pcr, qrt pcr, real time, real time per, thermal cycler, longgene, longGene.com.tr, ivd ce, biotechnology, LongGene europe, europe pcr, disgnostics, snp, absolute quantification, relative quantification, dna, cdna, thermal protocol, LongGene, long gene, end point pcr, covid19, fast pcr, polymerase chain reaction, Q1000, Q1000+, optimal, longgene turkey, LongGene europe, pcr mix, rt-pcr mix, how to prepare pcr mix, 16 well rt pcr, optimal software\" class=\"wp-image-929\" srcset=\"https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R2-1024x530.jpg 1024w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R2-300x155.jpg 300w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R2-768x398.jpg 768w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R2-1536x795.jpg 1536w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R2-1000x518.jpg 1000w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R2-1800x932.jpg 1800w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R2-1082x560.jpg 1082w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R2-600x311.jpg 600w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R2.jpg 1920w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><figcaption>Create your protocol according to SyberGreen master mix protocol <\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"1024\" height=\"582\" src=\"https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R3-1024x582.jpg\" alt=\"q2000, q2000a, q2000b, q2000c, pcr, qpcr, rt pcr, qrt pcr, real time, real time per, thermal cycler, longgene, longGene.com.tr, ivd ce, biotechnology, LongGene europe, europe pcr, disgnostics, snp, absolute quantification, relative quantification, dna, cdna, thermal protocol, LongGene, long gene, end point pcr, covid19, fast pcr, polymerase chain reaction, Q1000, Q1000+, optimal, longgene turkey, LongGene europe, pcr mix, rt-pcr mix, how to prepare pcr mix, 16 well rt pcr, optimal software\" class=\"wp-image-930\" srcset=\"https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R3-1024x582.jpg 1024w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R3-300x171.jpg 300w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R3-768x437.jpg 768w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R3-1536x874.jpg 1536w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R3-1000x569.jpg 1000w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R3-985x560.jpg 985w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R3-600x341.jpg 600w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R3.jpg 1744w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><figcaption>Create two target with SYBR dye (Your target GEN &amp; GAPDH)<\/figcaption><\/figure>\n\n\n\n<ul><li>Create <strong>two targets <\/strong>with the <strong>same<\/strong> SYBR DYE; Name your GENE (red) and REFERENCE gene (Green: GAPDH or Beta Actin) <\/li><li>Use <strong>2 (two) separate reaction tubes<\/strong> with the same sample name <strong>for each samples<\/strong> ;<ul><li>Sample 1: Your TARGET GENE (Your target gene primers must be use in this reaction tube) <\/li><li>Sample 1 : Your reference gene GAPDH (Reference gene primers must be used in this reaction tube) <\/li><\/ul><\/li><li>Select your control sample and reference target (You can change them after the run completed if it need)<\/li><li>RUN your protocol.. <\/li><\/ul>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"1024\" height=\"533\" src=\"https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R4-1024x533.jpg\" alt=\"q2000, q2000a, q2000b, q2000c, pcr, qpcr, rt pcr, qrt pcr, real time, real time per, thermal cycler, longgene, longGene.com.tr, ivd ce, biotechnology, LongGene europe, europe pcr, disgnostics, snp, absolute quantification, relative quantification, dna, cdna, thermal protocol, LongGene, long gene, end point pcr, covid19, fast pcr, polymerase chain reaction, Q1000, Q1000+, optimal, longgene turkey, LongGene europe, pcr mix, rt-pcr mix, how to prepare pcr mix, 16 well rt pcr, optimal software\" class=\"wp-image-931\" srcset=\"https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R4-1024x533.jpg 1024w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R4-300x156.jpg 300w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R4-768x400.jpg 768w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R4-1536x799.jpg 1536w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R4-1000x520.jpg 1000w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R4-1800x937.jpg 1800w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R4-1076x560.jpg 1076w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R4-600x312.jpg 600w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R4.jpg 1918w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><figcaption>Select Amplification for Amplification Plots<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"1024\" height=\"531\" src=\"https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R5-1024x531.jpg\" alt=\"q2000, q2000a, q2000b, q2000c, pcr, qpcr, rt pcr, qrt pcr, real time, real time per, thermal cycler, longgene, longGene.com.tr, ivd ce, biotechnology, LongGene europe, europe pcr, disgnostics, snp, absolute quantification, relative quantification, dna, cdna, thermal protocol, LongGene, long gene, end point pcr, covid19, fast pcr, polymerase chain reaction, Q1000, Q1000+, optimal, longgene turkey, LongGene europe, pcr mix, rt-pcr mix, how to prepare pcr mix, 16 well rt pcr, optimal software\" class=\"wp-image-932\" srcset=\"https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R5-1024x531.jpg 1024w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R5-300x156.jpg 300w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R5-768x398.jpg 768w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R5-1536x797.jpg 1536w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R5-1000x519.jpg 1000w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R5-1800x934.jpg 1800w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R5-1079x560.jpg 1079w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R5-600x311.jpg 600w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R5.jpg 1916w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><figcaption>Select Gene Expression to complete your Relative Quantification<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"1024\" height=\"532\" src=\"https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R6-1024x532.jpg\" alt=\"q2000, q2000a, q2000b, q2000c, pcr, qpcr, rt pcr, qrt pcr, real time, real time per, thermal cycler, longgene, longGene.com.tr, ivd ce, biotechnology, LongGene europe, europe pcr, disgnostics, snp, absolute quantification, relative quantification, dna, cdna, thermal protocol, LongGene, long gene, end point pcr, covid19, fast pcr, polymerase chain reaction, Q1000, Q1000+, optimal, longgene turkey, LongGene europe, pcr mix, rt-pcr mix, how to prepare pcr mix, 16 well rt pcr, optimal software\" class=\"wp-image-933\" srcset=\"https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R6-1024x532.jpg 1024w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R6-300x156.jpg 300w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R6-768x399.jpg 768w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R6-1536x798.jpg 1536w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R6-1000x519.jpg 1000w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R6-1800x935.jpg 1800w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R6-1078x560.jpg 1078w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R6-600x312.jpg 600w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R6.jpg 1918w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><figcaption>You can change the Scale if it needs<\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" width=\"1024\" height=\"544\" src=\"https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R7-1024x544.jpg\" alt=\"q2000, q2000a, q2000b, q2000c, pcr, qpcr, rt pcr, qrt pcr, real time, real time per, thermal cycler, longgene, longGene.com.tr, ivd ce, biotechnology, LongGene europe, europe pcr, disgnostics, snp, absolute quantification, relative quantification, dna, cdna, thermal protocol, LongGene, long gene, end point pcr, covid19, fast pcr, polymerase chain reaction, Q1000, Q1000+, optimal, longgene turkey, LongGene europe, pcr mix, rt-pcr mix, how to prepare pcr mix, 16 well rt pcr, optimal software\" class=\"wp-image-934\" srcset=\"https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R7-1024x544.jpg 1024w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R7-300x159.jpg 300w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R7-768x408.jpg 768w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R7-1536x816.jpg 1536w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R7-1000x531.jpg 1000w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R7-1800x956.jpg 1800w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R7-1054x560.jpg 1054w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R7-600x319.jpg 600w, https:\/\/longgene.com.tr\/wp-content\/uploads\/2021\/10\/R7.jpg 1920w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><figcaption>You can select RQ Sample or RQ Dye<\/figcaption><\/figure>\n\n\n\n<p><strong>Related Links;<\/strong><\/p>\n\n\n\n<hr class=\"wp-block-separator is-style-wide\"\/>\n\n\n\n<div class=\"wp-block-buttons\">\n<div class=\"wp-block-button has-custom-font-size has-small-font-size\"><a class=\"wp-block-button__link\" href=\"https:\/\/longgene.com.tr\/?p=672\">How to install Optimal Software to your computer?<\/a><\/div>\n\n\n\n<div class=\"wp-block-button has-custom-font-size has-small-font-size\"><a class=\"wp-block-button__link\" href=\"https:\/\/longgene.com.tr\/?p=531\">How to create a protocol with Optimal Software?<\/a><\/div>\n\n\n\n<div class=\"wp-block-button has-custom-font-size has-small-font-size\"><a class=\"wp-block-button__link\" href=\"https:\/\/longgene.com.tr\/?p=554\">How to export your result with Optimal Software?<\/a><\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Gene expression analysis by quantitative&nbsp;reverse transcription PCR&nbsp;(qRT\u2013PCR) allows accurate quantifications of messenger RNA (mRNA) levels over different samples. Create two targets with the same SYBR DYE; Name your GENE (red) and REFERENCE gene (Green: GAPDH or Beta Actin) Use 2 (two) separate reaction tubes with the same sample name for [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":[],"categories":[5,7,23],"tags":[9,10,41,42,43,44],"_links":{"self":[{"href":"https:\/\/longgene.com.tr\/index.php?rest_route=\/wp\/v2\/posts\/926"}],"collection":[{"href":"https:\/\/longgene.com.tr\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/longgene.com.tr\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/longgene.com.tr\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/longgene.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=926"}],"version-history":[{"count":5,"href":"https:\/\/longgene.com.tr\/index.php?rest_route=\/wp\/v2\/posts\/926\/revisions"}],"predecessor-version":[{"id":945,"href":"https:\/\/longgene.com.tr\/index.php?rest_route=\/wp\/v2\/posts\/926\/revisions\/945"}],"wp:attachment":[{"href":"https:\/\/longgene.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=926"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/longgene.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=926"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/longgene.com.tr\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=926"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}