Gene expression analysis by quantitative reverse transcription PCR (qRT–PCR) allows accurate quantifications of messenger RNA (mRNA) levels over different samples. Create two targets with the same SYBR DYE; Name your GENE (red) and REFERENCE gene (Green: GAPDH or Beta Actin) Use 2 (two) separate reaction tubes with the same sample name for […]
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How to check the performance of the thermal gradient block?
Real time PCR instruments has two main functions; thermal block and optical module, new generation peltiers control the heating and cooling sensitivity, LED excitation source and new generation CCD cameras are stated in the optical module. So, to check the performance of a RT-PCR; you have to check the performance […]
Read MoreWhat could be the reason of poor RT-PCR results?
Achieving accurate and reliable results depends on: diagnostic kit, RNA prep solution, swap for sampling, disposables (pipette tips, strip tubes and plates for RT-PCR) and instrument. Possible Reasons of Poor Results; Not to store IVD kit at -20 C in a deep freezer (Please check the expiration date) To use […]
Read MoreHow to do a self-test to check if there is a problem?
Achieving accurate and reliable results depends on: diagnostic kit, RNA prep solution, swap for sampling, disposables (pipette tips, strip tubes and plates for RT-PCR) and instrument. Outstanding Features of the LongGene devices Using LED light sources for each channel separately (No optical calibration is needed) Using new generation SSLP CCD […]
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Service Forms
Two option can be used to inform your problem to our after sales service support team, your can fill the below pdf form completely and please send the scanned copy to our service e-mail. Or you can fill the online form to send it without sending an e-mail. As written […]
Read MoreHow to set ip address of computer to use Optimal Software?
To be able to use optimal control and analysis software, ip settings of the computer has to be done (no need to change ip of the instrument), please close the Wi/Fi (or all other internet connections) before starting this process, please note that Wi-Fi must be turned off while the […]
Read MoreHow to install Optimal Software to your computer?
To be able to use optimal control and analysis software, ip settings of the instrument has to be done, please close the Wi/Fi (or all other internet connections) before starting this process, please note that Wi-Fi must be turned off while the device is running as well; Optimal software can […]
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