Achieving accurate and reliable results depends on: diagnostic kit, RNA prep solution, swap for sampling, disposables (pipette tips, strip tubes and plates for RT-PCR) and instrument. Possible Reasons of Poor Results; Not to store IVD kit at -20 C in a deep freezer (Please check the expiration date) To use […]
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How to interpret the RT-PCR results ?
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Read MoreRT-PCR Result: No Amplification
To check whether no amplification is caused by kir or instrument; Deselect the Subtract Baseline and Normalization functions If the lines are unexpected curves as above then it proves that instrument is collection data so you have to focus on your protocol or kits If the there is only a […]
Read MoreRT-PCR Result: Lower Amplification
Low amplification plots may be caused by; Low quality PCR strip tubes, optical tubes, plates or sealing may be used Master mix may be melted many times (more than 3 times), efficiency may be down Kits may be out of date, please check the expiration dates of the RNA isolation […]
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