LongGene Optimal is a functional control and analysis software that you can use it to create and use your protocols, analysis your results, also export your run datas. Related Links;
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How to create a protocol with Optimal Software?
Optimal uses the special *.txc files, you can create a new one or you can use Template button to select your created (ready to use) protocols for the quick setup. Please close the connection between computer and instrument to be able to create a ready to use protocol correctly. After […]
Read MoreDownload Optimal Software and ‘txc’ Files
You can download and install the newest Optimal Software versions from this page, also you can download and use mostly used protocols in txc format to be able to open and use it with Optimal, please note that the files ending with 48 are compatible with Q1000 series (48 Well) […]
Read MoreHow to interpret the RT-PCR results ?
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Read MoreHow is the RT-PCR Mix prepared?
Pipetting Master Mixes and Samples New generation diagnostic kits are using 1-Step RT-PCR master mixes which includes the cDNA enzime to be able to complete the PCR protocol as a single RUN, without any additional steps, without any interruption. Almost all kits has a single master mix tubes, PC and […]
Read MoreRT-PCR Result: No Amplification
To check whether no amplification is caused by kir or instrument; Deselect the Subtract Baseline and Normalization functions If the lines are unexpected curves as above then it proves that instrument is collection data so you have to focus on your protocol or kits If the there is only a […]
Read MoreRT-PCR Result: Lower Amplification
Low amplification plots may be caused by; Low quality PCR strip tubes, optical tubes, plates or sealing may be used Master mix may be melted many times (more than 3 times), efficiency may be down Kits may be out of date, please check the expiration dates of the RNA isolation […]
Read MoreRT-PCR Result: Normal Amplification
This is an expected Real Time PCR results because; (FAM: Specific regions for Covid-19 virus, HEX: Internal Control, RP gene for extraction) Positive control (PC) is amplified normally (This proves that PCR is done, instrument and kit is working properly) Negative Control (NC) has no amplification, so it proves that […]
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